How a Sandwich Lateral Flow Assay Works
Watch whole blood become a readable result as plasma, analyte and labelled antibodies move through a lateral flow strip.
- Duration
- 62s
Interactive animation
Use the play button or timeline to inspect the mechanism at your own pace.
Explanation
How a Sandwich Lateral Flow Assay Works
A sandwich lateral flow test separates plasma, releases a labelled detection antibody and captures the analyte between two antibodies at the test line. The control line independently confirms that liquid and conjugate travelled through the strip.
Why it matters
Every visible line is the result of coordinated sample preparation, transport, binding and wash-through. Understanding those steps makes troubleshooting and assay design more systematic.
Key takeaway
A positive test line forms only when analyte creates an antibody sandwich; the control line proves the conjugate completed the run.