HbA1c by Boronate Fluorescence Quenching
See how boronic acid binds glycated haemoglobin, quenches fluorescence and turns a molecular interaction into an HbA1c result.
- Duration
- 40s
Interactive animation
Use the play button or timeline to inspect the mechanism at your own pace.
Explanation
HbA1c by Boronate Fluorescence Quenching
Fluorescent boronic acid conjugates bind the cis-diol on glycated haemoglobin. Binding places the fluorophore near haem groups and reduces its emission. The fluorescence drop estimates glycated haemoglobin, while a separate absorbance measurement estimates total haemoglobin.
Why it matters
This method converts glycation into an optical ratio rather than separating haemoglobin variants. Its performance depends on binding chemistry, optical calibration and an accurate total-haemoglobin measurement.
Key takeaway
More glycated haemoglobin binds more conjugate and produces a larger fluorescence drop; normalization to total haemoglobin yields HbA1c.