Setting the cutoff
four levers on the test line
1 Capture
2 Conjugate
3 Flow
4 Scavenger
Top view
Sample pad
Conjugate
Wick
T
C
scavenger
Cross section
Scavenger antibody
Test line (T)
Control line (C)
flow
Where the T line turns visible
Analyte concentration →
T line signal →
this sample
visible threshold
cutoff
Pause
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